This antibody recognises cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. Clone 4C2 is highly specific for cytoplasmic actin ß and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodeling.
Applications
WB, IF, IHC-Fr
Recommander des dilutions
IHC-Fr: 1:50 - 1:100
Reactivité
Human, Rabbit, Pig, Mouse, Rat
Immunogène
Synthetic peptide corresponding to the acetylated N-terminal nonapeptide of beta-cytoplasmic actin, coupled to Keyhole Limpet Haemocyanin.
Hôte
Mouse
Clonalité
Monoclonal
Clone
4C2
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Stockage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Notes générales
Mouse anti Human actin beta, clone 4C2, recognizes cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kDa cytoskeletal protein.Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals (Vandekerckhove et al. 1978). Clone 4C2 is highly specific for cytoplasmic actin ß and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following sendai virus infection and the specific role of the viral M protein in actin remodeling (Miazza et al. 2011).