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Anti-beta Catenin Anticorps [ARC0136] (A307069)

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Nom du produit
Anti-beta Catenin Antibody [ARC0136]
Description du produit
Rabbit monoclonal [ARC0136] antibody to beta Catenin.
Applications
WB, IHC, ICC/IF, IP
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (P35222).
Séquence
MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0136
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
92 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Beta-catenin, Cadherin associated protein, Catenin (cadherin associated protein), beta 1, 88kDa, Catenin beta 1, Catenin beta-1, CATNB, CHBCAT, CTNB1_HUMAN, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, OTTHUMP00000162082, OTTHUMP00000165222, OTTHUMP00000165223, OTTHUMP00000209288, OTTHUMP00000209289
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of HeLa cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts of Mouse brain, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Western Blot - Anti-beta Catenin Antibody [ARC0136] (A307069)
Western blot analysis of extracts from wild type (WT) and beta-Catenin knockout (KO) 293T cells, using Anti-beta Catenin Antibody [ARC0136] (A307069) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-beta Catenin Antibody [ARC0136] (A307069)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-beta Catenin Antibody [ARC0136] (A307069) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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