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Anti-beta III Tubulin Anticorps [AMC0115] (A308612)

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Immunocytochemistry - Anti-beta III Tubulin Antibody [TU-20] (A86689) - Antibodies.com
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Anti-beta III Tubulin Anticorps [TU-20]
Anticorps monoclonal [TU-20] de souris contre beta III Tubulin pour Flow Cytometry (Intracellular), WB, IHC-P et ICC.
Immunocytochemistry - Anti-beta III Tubulin Antibody [TU-20] (A86689) - Antibodies.com
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Anti-beta III Tubulin Anticorps [TU-20]
Anticorps monoclonal [TU-20] de souris contre beta III Tubulin pour Flow Cytometry (Intracellular), WB, IHC-P et ICC.
Nom du produit
Anti-beta III Tubulin Antibody [AMC0115]
Description du produit
Mouse monoclonal [AMC0115] antibody to beta III Tubulin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (Q13509).
Séquence
SDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Hôte
Mouse
Clonalité
Monoclonal
Clone
AMC0115
Isotype
IgG2b
Chaînes légères
kappa
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
55 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
beta 3 tubulin, beta-4, CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, M(beta)3, M(beta)6, MC1R, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, QccE-11995, QccE-15186, TBB3_HUMAN, Tubb 3, TUBB3, TUBB4, Tubulin beta 3, Tubulin beta 3 chain, Tubulin beta 4, Tubulin beta III, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-beta III Tubulin Antibody [AMC0115] (A308612) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-beta III Tubulin Antibody [AMC0115] (A308612)
Western blot analysis of extracts from wild type(WT) and betaIII-Tubulin knockdown (KD) HeLa cells, using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-beta III Tubulin Antibody [AMC0115] (A308612)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at dilution of 100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-beta III Tubulin Antibody [AMC0115] (A308612) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-beta III Tubulin Antibody [AMC0115] (A308612)
Immunofluorescence analysis of mouse brain cells using Anti-beta III Tubulin Antibody [AMC0115] (A308612) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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