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Anti-beta Tubulin Anticorps (A80770)

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Anti-beta Tubulin Anticorps [TU-13]
Anticorps monoclonal [TU-13] de souris contre beta Tubulin pour WB, IHC-Fr et ICC.
Nom du produit
Anti-beta Tubulin Antibody
Description du produit
Rabbit polyclonal antibody to beta Tubulin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human ß-Tubulin (NP_006077.2).
Séquence
SDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
50 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-beta Tubulin Antibody (A80770) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-beta Tubulin Antibody (A80770)
Western blot analysis of extracts of various cell lines, using Anti-beta Tubulin Antibody (A80770) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-beta Tubulin Antibody (A80770) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-beta Tubulin Antibody (A80770)
Immunohistochemistry analysis of paraffin-embedded HepG2 cells using Anti-beta Tubulin Antibody (A80770) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-beta Tubulin Antibody (A80770) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-beta Tubulin Antibody (A80770)
Immunohistochemistry analysis of paraffin-embedded HepG2 cells treated by oleic acid using Anti-beta Tubulin Antibody (A80770) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-beta Tubulin Antibody (A80770) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-beta Tubulin Antibody (A80770)
Immunohistochemistry analysis of paraffin-embedded HepG2 cells treated by oleic acid using Anti-beta Tubulin Antibody (A80770) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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