Anti-CACNA1H Anticorps [N55/10] (A304750)

Name: Anti-CACNA1H Antibody [N55/10]
Brand: Antibodies.com
SKU: A304750
Price: 500.0 USD
Availability: InStock

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$500

Anticorps monoclonal [N55/10] de souris contre CACNA1H pour WB, IHC, ICC/IF, IP et Antibody Microarray.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-CACNA1H Antibody [N55/10]

Description du produit

Mouse monoclonal [N55/10] antibody to CACNA1H.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Recommander des dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Réactivité croisée

This antibody does not cross-react with Cav1.3.

Immunogène

Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2.

Hôte

Mouse

Clonalité

Monoclonal

Clone

N55/10

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Masse moléculaire

~260 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This antibody was formely sold under the Clone ID S55-10.

Synonymes

Alpha1 3.2, Alpha13.2, CAC1H_HUMAN, CACNA 1H, CACNA1 H, CACNA1 HB, CACNA1HB, Calcium channel alpha13.2 subunit, Calcium channel voltage dependent T type alpha 1H subunit, Calcium channel, voltage-dependent, T type, alpha 1Hb subunit, Cav 3.2, Cav T.2, Cav3.2, CavT.2, EIG 6, EIG6, Low voltage activated calcium channel alpha 13.2 subunit, Low voltage activated calcium channel alpha1 3.2 subunit, Low-voltage-activated calcium channel alpha1 3.2 subunit, MNCb 1209, T type Cav3.2, Voltage dependent t type calcium channel alpha 1H subunit, Voltage gated calcium channel alpha subunit Cav 3.2, Voltage gated calcium channel alpha subunit Cav T.2, Voltage gated calcium channel alpha subunit Cav3.2, Voltage gated calcium channel alpha subunit CavT.2, Voltage-dependent T-type calcium channel subunit alpha-1H, Voltage-gated calcium channel subunit alpha Cav3.2

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-CACNA1H Antibody [N55/10] (A304750)

Western blot analysis of rat brain membrane lysate (native) showing detection of ~261 kDa Cav3.2 protein using Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 2 hours at room temperature. Block: 2% Skim Milk + 2% BSA in TBST. The secondary antibody used was Anti-Mouse: HRP at 1:4000. Predicted/Observed Size: ~261 kDa.

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Figure 2: Immunofluorescence - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-CACNA1H Antibody [N55/10] (A304750), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.2 Antibody (D) Composite.

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Figure 3: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human frozen brain section, fixed in 10% formalin solution for 12-24 hours at room temperature. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at room temperature.

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Figure 4: Immunohistochemistry - Anti-CACNA1H Antibody [N55/10] (A304750)

Immunohistochemistry analysis of human hippocampus, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CACNA1H Antibody [N55/10] (A304750) at 1:1,000 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature.