Anti-Calpain 1 Anticorps (A13348)

Name: Anti-Calpain 1 Antibody
Brand: Antibodies.com
SKU: A13348
Price: 335.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$335

Anticorps polyclonal de lapin contre Calpain 1 pour WB, IHC et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-Calpain 1 Antibody

Description du produit

Rabbit polyclonal antibody to Calpain 1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 355-530 of human Calpain 1 (NP_005177.2).

Séquence

PDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQIQA

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

75 - 80 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Ca2 activated neutral protease, Calcium activated neutral proteinase, Calcium activated neutral proteinase small subunit, Calcium dependent protease small subunit, Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain 1, (mu/I) large subunit, Calpain mu type, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, Calpain-1 large subunit, CAN1_HUMAN, CANP, CANP 1, CANP small subunit, CANP1, CANPL 1, CANPL1, CAPN 1, CAPN1, Cell proliferation inducing protein 30, Cell proliferation-inducing gene 30 protein, Micromolar Calpain, Micromolar-calpain, Mu Calpain, muCANP, muCL

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-Calpain 1 Antibody (A13348)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 1 Antibody (A13348) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunohistochemistry - Anti-Calpain 1 Antibody (A13348)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Calpain 1 Antibody (A13348) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunofluorescence - Anti-Calpain 1 Antibody (A13348)

Immunofluorescence analysis of HeLa cells using Anti-Calpain 1 Antibody (A13348) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunofluorescence - Anti-Calpain 1 Antibody (A13348)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Calpain 1 Antibody (A13348) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).