Anti-Calreticulin Anticorps [ARC51269] (A305767)

Name: Anti-Calreticulin Antibody [ARC51269]
Brand: Antibodies.com
SKU: A305767

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Ce produit est discontinué

Anti-Calreticulin Anticorps [ARC51269] (A305767) a été abandonné et n'est plus disponible.

Voir toutes Anti-Calreticulin Anticorps.

Spécifications

Nom du produit

Anti-Calreticulin Antibody [ARC51269]

Description du produit

Rabbit monoclonal [ARC51269] antibody to Calreticulin.

Applications

WB, IHC

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 18-203 of human Calreticulin (NP_004334.1).

Séquence

EPAVYFKEQFLDGDGWTSRWIESKHKSDFGKFVLSSGKFYGDEEKDKGLQTSQDARFYALSASFEPFSNKGQTLVVQFTVKHEQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFNYKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDDWDFL

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC51269

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

55 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Autoantigen RO, CALR, CALR protein, Calregulin, CALR_HUMAN, cC1qR, CRP55, CRT, CRTC, Endoplasmic reticulum resident protein 60, Epididymis secretory sperm binding protein Li 99n, ERp60, FLJ26680, grp60, HACBP, HEL S 99n, RO, Sicca syndrome antigen A, Sicca syndrome antigen A (autoantigen Ro; calreticulin), SSA

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (8)

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Figure 1: Western Blot - Anti-Calreticulin Antibody [ARC51269] (A305767)

Western blot analysis of extracts of various cell lines, using Anti-Calreticulin Antibody [ARC51269] (A305767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Calreticulin Antibody [ARC51269] (A305767)

Western blot analysis of extracts from wild type (WT) and PD-1/CD279 knockdown (KD) 293T cells, using Anti-Calreticulin Antibody [ARC51269] (A305767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-Calreticulin Antibody [ARC51269] (A305767)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Calreticulin Antibody [ARC51269] (A305767) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Calreticulin Antibody [ARC51269] (A305767)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Calreticulin Antibody [ARC51269] (A305767) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.