Anti-CaMKII Anticorps [22B1] (A305070)

Name: Anti-CaMKII Antibody [22B1]
Brand: Antibodies.com
SKU: A305070
Price: 465.0 USD
Availability: InStock

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$465

Anticorps monoclonal [22B1] de souris contre CaMKII pour WB, IHC, ICC/IF, IP et ELISA.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Spécifications

Nom du produit

Anti-CaMKII Antibody [22B1]

Description du produit

Mouse monoclonal [22B1] antibody to CaMKII.

Spécificité

This antibody detects phosphorylated CaMKII from rat tissues. This antibody is specific for a and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).

Applications

WB, IHC, ICC/IF, IP, ELISA

Recommander des dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

Synthetic peptide to Rat CaMKII.

Hôte

Mouse

Clonalité

Monoclonal

Clone

22B1

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Calcium/calmodulin dependent protein kinase II alpha, Calcium/calmodulin dependent protein kinase II beta, Calcium/calmodulin dependent protein kinase II delta, Calcium/calmodulin dependent protein kinase II gamma, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II, CaM kinase II alpha, CaM kinase II beta, CaM kinase II delta, CaM kinase II gamma, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2, Camk2a, CAMK2B, CAMK2D, CAMK2G, CAMKA, KCC2A_HUMAN

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-CaMKII Antibody [22B1] (A305070)

Western blot analysis of mouse Ventricle lysates showing detection of CaMKII protein using Anti-CaMKII Antibody [22B1] (A305070) at 1:1,000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.

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Figure 2: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:5,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x.

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Figure 3: Immunohistochemistry - Anti-CaMKII Antibody [22B1] (A305070)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-CaMKII Antibody [22B1] (A305070) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.

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Figure 4: Immunofluorescence - Anti-CaMKII Antibody [22B1] (A305070)

Immunocytochemistry/Immunofluorescence analysis of rat dissociated hippocampal neurons, fixed in cold 4% paraformaldehyde / 0.2% glutaraldehyde in 0.1M sodium phosphate buffer, using Anti-CaMKII Antibody [22B1] (A305070), at 1:1,000 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at room temperature. Magnification: 10X.