This antibody recognises human caspase-2, a 53 kDa member of the cysteine-aspartic acid protease (caspase) family. Caspase-2 is required for the permeabilisation of the mitochondria and the resulting amplification of caspase activity, in response to cytotoxic stress. Cytotoxic stress induced proteolytic cleavage of the caspase-2 proenzyme forms an active caspase which cleaves other caspases in a cascade. This leads to the release of cytochrome c and Smac from mitochondria and the translocation of Bax from the cytoplasm to the mitochondria, leading to apoptosis. In adults, caspase-2 is expressed in the placenta, lung, kidney, pancreas and at lower levels in the heart, brain, liver and skeletal muscle.

Applications

WB, IHC-P

Recommander des dilutions

IHC-P 1: 10 µg/ml, WB: 1 µg/ml

Reactivité

Human, Mouse

Immunogène

Synthetic peptide corresponding to 6 amino acids near the N-terminus of human Caspase-2.

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Stockage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Notes générales

recognises human caspase-2, a 53 kDa member of the cysteine-aspartic acid protease (caspase) family. Caspase-2 is required for the permeabilisation of the mitochondria and the resulting amplification of caspase activity, in response to cytotoxic stress. Cytotoxic stress induced proteolytic cleavage of the caspase-2 proenzyme forms an active caspase which cleaves other caspases in a cascade. This leads to the release of cytochrome c and Smac from mitochondria and the translocation of Bax from the cytoplasm to the mitochondria, leading to apoptosis. In adults, caspase-2 is expressed in the placenta, lung, kidney, pancreas and at lower levels in the heart, brain, liver and skeletal muscle.

Synonymes

CASP 2, CASP-2, Casp2, CASP2_HUMAN, Caspase 2, Caspase 2 apoptosis related cysteine peptidase, Caspase-2 subunit p12, Caspase2, ICH 1, ICH 1 protease, ICH 1L, ICH1, ICH1 protease, ICH1L, NEDD-2, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2, PPP1R57, Protease ICH-1, Protein phosphatase 1 regulatory subunit 57

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.