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CBX5 Cell Based Kit Elisa (A103513)

Nom du produit
CBX5 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This CBX5 Cell Based ELISA Kit allows for the detection of CBX5 and the effects that certain stimulation conditions have on CBX5 expression in different cell lines. Qualitative determination of CBX5 concentration is achieved by an indirect ELISA format. In essence, the CBX5 is captured by Anti-CBX5 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CBX5 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
Antigen p25, CBX5_HUMAN, CG8409, Chromobox 5, Chromobox homolog 5, Chromobox homolog 5 (HP1 alpha homolog, Drosophila), Chromobox protein homolog 5, Epididymis luminal protein 25, HEL25, Heterochromatin protein 1, Heterochromatin protein 1 alpha, Heterochromatin protein 1 homolog alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1Hs alpha, Su(var)205
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - CBX5 Cell Based ELISA Kit (CB5894) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - CBX5 Cell Based ELISA Kit (CB5894)
Western blot analysis of extracts from HeLa/A549/K562 cells, using Anti-CBX5 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - CBX5 Cell Based ELISA Kit (CB5894) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: CBX5 Cell Based ELISA Kit (CB5894)
A diagram showing the protocol for CBX5 Cell Based ELISA Kit.
Protocol Summary - CBX5 Cell Based ELISA Kit (CB5894) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: CBX5 Cell Based ELISA Kit (CB5894)
A summary of the protocol for CBX5 Cell Based ELISA Kit.
Western Blot - Control Antibody - CBX5 Cell Based ELISA Kit (CB5894) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - CBX5 Cell Based ELISA Kit (CB5894)
The mouse monoclonal antibody to GAPDH is used as a positive control inCBX5 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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