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Anti-CD13 Anticorps [ARC0670] (A80859)

Nom du produit
Anti-CD13 Antibody [ARC0670]
Description du produit
Rabbit monoclonal [ARC0670] antibody to CD13.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CD13/ANPEP (P15144).
Séquence
NAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALA
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0670
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
160 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13 antigen, gp150, hAPN, LAP 1, LAP1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD13 Antibody [ARC0670] (A80859)
Western blot analysis of extracts of various cell lines, using Anti-CD13 Antibody [ARC0670] (A80859) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-CD13 Antibody [ARC0670] (A80859)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859)
Immunofluorescence analysis of THP-1 cells using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-CD13 Antibody [ARC0670] (A80859)
Immunofluorescence analysis of mouse kidney using Anti-CD13 Antibody [ARC0670] (A80859) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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