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Anti-CD133 Anticorps (A12549)

Western Blot - Anti-CD133 Antibody (A12549) - Antibodies.com Western Blot - Anti-CD133 Antibody (A12549) - Antibodies.com Immunofluorescence - Anti-CD133 Antibody (A12549) - Antibodies.com Immunofluorescence - Anti-CD133 Antibody (A12549) - Antibodies.com
$335
Product Datasheet

Anticorps polyclonal de lapin contre CD133 pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-CD133 Antibody
Description du produit
Rabbit polyclonal antibody to CD133.
Applications
WB, IHC, ICC/IF, IP
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1).
Séquence
FEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDH
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
120 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
AC133, Antigen AC133, CORD12, Hematopoietic stem cell antigen, hProminin, MCDR2, MSTP061, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, PROM1_HUMAN, Prominin I, Prominin like 1, Prominin like protein 1 precursor, Prominin mouse like 1, Prominin-1, Prominin-like protein 1, Prominin1, PROML1, RP41, STGD4
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD133 Antibody (A12549) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD133 Antibody (A12549)
Western blot analysis of extracts of HCT116 cells, using Anti-CD133 Antibody (A12549) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-CD133 Antibody (A12549) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CD133 Antibody (A12549)
Western blot analysis of extracts of various cell lines, using Anti-CD133 Antibody (A12549) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-CD133 Antibody (A12549) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-CD133 Antibody (A12549)
Immunofluorescence analysis of NIH/3T3 cells using Anti-CD133 Antibody (A12549) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD133 Antibody (A12549) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-CD133 Antibody (A12549)
Immunofluorescence analysis of U-2 OS cells using Anti-CD133 Antibody (A12549) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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