This antibody recognises the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

Applications

WB, IHC-P, IP, Flow Cytometry, IHC-Fr, ELISA, IF

Recommander des dilutions

Flow Cytometry: 1:50 - 1:100, IHC-P: 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Reactivité

Human

Hôte

Mouse

Clonalité

Monoclonal

Clone

11G5a

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Stockage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Notes générales

Mouse anti Human CD151 antibody, clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen (Crew et al. 2004).It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and co-immunoprecipitation may occur even in quite stringent conditions (Yauch. et al. 1998).

Synonymes

CD151 antigen, CD151 molecule, CD151 molecule (Raph blood group), CD151_HUMAN, GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, PETA-3, PETA3, Platelet endothelial tetraspan antigen 3, platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA 1, SFA1, Tetraspanin-24, Tspan-24, TSPAN24

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.