Anti-CD20 Anticorps [B9E9] (A253773)

Anti-CD20 Antibody [B9E9] - BSA and Azide free

Mouse monoclonal [B9E9] antibody to CD20.

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 This antibody recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

Functional Studies, Flow Cytometry, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

Lymphoblastoid cell line Daudi.

Mouse

Monoclonal

B9E9

IgG2a

kappa

Protein A/G chromatography.

1 mg/ml

33-37 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-CD20 Antibody [B9E9] (A250593).

APY, ATOPY, B lymphocyte antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, B1, Bp 35, Bp35, CD 20, CD20 antigen, CD20 receptor, CD20_HUMAN, CVID5, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU 16, LEU16, leukocyte surface antigen Leu 16, Leukocyte surface antigen Leu-16, Ly44, Membrane spanning 4 domains A1, Membrane spanning 4 domains subfamily A member 2, Membrane-spanning 4-domains subfamily A member 1, MGC3969, MS4A1, MS4A2, S7