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Anti-CD45 Anticorps (A13883)

Western Blot - Anti-CD45 Antibody (A13883) - Antibodies.com Immunohistochemistry - Anti-CD45 Antibody (A13883) - Antibodies.com Immunohistochemistry - Anti-CD45 Antibody (A13883) - Antibodies.com Immunofluorescence - Anti-CD45 Antibody (A13883) - Antibodies.com
$335
Product Datasheet

Anticorps polyclonal de lapin contre CD45 pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

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Nom du produit
Anti-CD45 Antibody
Description du produit
Rabbit polyclonal antibody to CD45.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 26-577 of human CD45 (P08575).
Séquence
QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSK
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
180 - 250 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
B220, CD 45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, loc, Ly-5, LY5, Ly5, homolog of, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, Protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD45 Antibody (A13883) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD45 Antibody (A13883)
Western blot analysis of various lysates, using Anti-CD45 Antibody (A13883) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD45 Antibody (A13883) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-CD45 Antibody (A13883)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD45 Antibody (A13883) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD45 Antibody (A13883) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CD45 Antibody (A13883)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD45 Antibody (A13883) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD45 Antibody (A13883) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-CD45 Antibody (A13883)
Immunofluorescence analysis of Jurkat cells using Anti-CD45 Antibody (A13883) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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