This antibody recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This antibody recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Applications

Functional Studies, Flow Cytometry, IF

Recommander des dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivité

Human

Réactivité croisée

This antibody does not cross react with Baboon or Horse.

Immunogène

Stimulated human leukocytes.

Hôte

Mouse

Clonalité

Monoclonal

Clone

161-2

Isotype

IgG2a

Chaînes légères

kappa

Conjuguer

Unconjugated

Purification

Protein A/G chromatography.

Concentration

200 µg/ml

Masse moléculaire

33-55 kDa

Forme du produit

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes générales

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD53 Antibody [161-2] - BSA and Azide free (A253918).

Synonymes

AI323659, Antigen MOX44 identified by monoclonal, CD 53, CD53 antigen, CD53 antigen, isoform CRA_b, CD53 glycoprotein, Cd53 molecule, CD53 tetraspan antigen, CD53_HUMAN, Cell surface glycoprotein CD53, Leukocyte antigen MRC OX-44, Leukocyte surface antigen CD53, MOX 44, MOX44, OTTHUMP00000059505, Ox-44, OX44, RATOX44, Tetraspanin 25, Tetraspanin-25, Tetraspanin25, Transmembrane glycoprotein, TSPAN 25, Tspan-25, TSPAN25

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (1)

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Figure 1: SDS-PAGE - Anti-CD53 Antibody [161-2] (A250738)

SDS-PAGE analysis of Anti-CD53 Antibody [161-2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.