Figure 1: Western Blot - Anti-CD68 Antibody [ARC51158] (A308974)
Western blot analysis of various lysates, using Anti-CD68 Antibody [ARC51158] (A308974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-CD68 Antibody [ARC51158] (A308974)
Western blot analysis of various lysates, using Anti-CD68 Antibody [ARC51158] (A308974) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD68 Antibody [ARC51158] (A308974) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD68 Antibody [ARC51158] (A308974) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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