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Anti-CD8 alpha Anticorps [ARC59319 + ARC55249] (A309931)

Nom du produit
Anti-CD8 alpha Antibody [ARC59319 + ARC55249]
Description du produit
Rabbit Oligoclonal [ARC59319 + ARC55249] antibody to CD8 alpha.
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:500-1:1,000
Reactivité
Human, Mouse
Immunogène
Recombinant protein of human/Mouse CD8A.
Hôte
Rabbit
Clonalité
Oligoclonal
Clone
ARC59319 + ARC55249
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
35 - 42 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931)
Western blot analysis of Mouse spleen, using Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.
Western Blot - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931)
Western blot analysis of various lysates, using Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD8 alpha Antibody [ARC59319 + ARC55249] (A309931) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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