Anti-CD8 Beta Anticorps [PPT23] (A281588)

Anti-CD8 Beta Antibody [PPT23]

Mouse monoclonal [PPT23] antibody to CD8 Beta.

This antibody recognises the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine a/ß T cells, in thymic tissue both CD8lo and CD8hi cells are recognized. recognises a different epitope on the CD8 beta chain to clone PPT22.

Flow Cytometry

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine thymus membrane lysate.

Mouse

Monoclonal

PPT23

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine a/ß T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22.