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Anti-CD81 Anticorps [ARC57928] (A309092)

Nom du produit
Anti-CD81 Antibody [ARC57928]
Description du produit
Rabbit monoclonal [ARC57928] antibody to CD81.
Applications
WB, IHC
Recommander des dilutions
WB: 1:1,000-1:5,000, IHC: 1:100-1:500
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 114-199 of human CD81 (NP_004347.1).
Séquence
VNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFS
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC57928
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
20 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
26 kDa cell surface protein TAPA 1, 26 kDa cell surface protein TAPA-1, 26 kDa cell surface protein TAPA1, CD 81, CD81 antigen, CD81 antigen (target of antiproliferative, CD81 molecule, CD81_HUMAN, CVID6, S5.7, TAPA 1, TAPA1, Target of the antiproliferative, Tetraspanin 28, Tetraspanin-28, Tetraspanin28, Tspan 28, Tspan-28, Tspan28
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-CD81 Antibody [ARC57928] (A309092) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-CD81 Antibody [ARC57928] (A309092)
Western blot analysis of various lysates, using Anti-CD81 Antibody [ARC57928] (A309092) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-CD81 Antibody [ARC57928] (A309092) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-CD81 Antibody [ARC57928] (A309092)
Western blot analysis of Rat brain, using Anti-CD81 Antibody [ARC57928] (A309092) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-CD81 Antibody [ARC57928] (A309092) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-CD81 Antibody [ARC57928] (A309092)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD81 Antibody [ARC57928] (A309092) at dilution of1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD81 Antibody [ARC57928] (A309092) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-CD81 Antibody [ARC57928] (A309092)
Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-CD81 Antibody [ARC57928] (A309092) at dilution of1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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