Anti-Cdk4 Anticorps (A309050)

Name: Anti-Cdk4 Antibody
Brand: Antibodies.com
SKU: A309050
Price: 470.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$470

Anticorps polyclonal de lapin contre Cdk4 pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-Cdk4 Antibody

Description du produit

Rabbit polyclonal antibody to Cdk4.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, IP: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 200-303 of human CDK4 (NP_000066.1).

Séquence

VGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

35 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Cdk 4, CDK4 protein, CDK4_HUMAN, Cell division kinase 4, Cell division protein kinase 4, CMM 3, CMM3, Crk3, Cyclin dependent kinase 4, Cyclin-dependent kinase 4, Melanoma cutaneous malignant 3, MGC14458, p34 cdk4, PSK J3, PSK-J3

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-Cdk4 Antibody (A309050)

Western blot analysis of extracts of various cell lines, using Anti-Cdk4 Antibody (A309050) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-Cdk4 Antibody (A309050)

Western blot analysis of extracts of 293T cells, using Anti-Cdk4 Antibody (A309050) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-Cdk4 Antibody (A309050)

Western blot analysis of extracts from wild type (WT) and CDK4 knockout (KO) HeLa cells, using Anti-Cdk4 Antibody (A309050) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-Cdk4 Antibody (A309050)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Cdk4 Antibody (A309050) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.