Rabbit polyclonal antibody to CDK6 (phospho-Tyr24)
Spécificité
The antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 24.
Applications
WB, IHC, IF
Reactivité
Human, Mouse, Rat
Immunogène
Peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y(p)-G-K) derived from Human CDK6.
Hôte
Rabbit
Clonalité
Polyclonal
Conjuguer
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Stockage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24) Antibody #11543 (left) or the same antibody preincubated with blocking peptide (right).