Anti-COL1A2 Anticorps [ARC52128] (A308262)

Name: Anti-COL1A2 Antibody [ARC52128]
Brand: Antibodies.com
SKU: A308262

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Ce produit est discontinué

Anti-COL1A2 Anticorps [ARC52128] (A308262) a été abandonné et n'est plus disponible.

Voir toutes Anti-COL1A2 Anticorps.

Spécifications

Nom du produit

Anti-COL1A2 Antibody [ARC52128]

Description du produit

Rabbit monoclonal [ARC52128] antibody to COL1A2.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivité

Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Collagen I/COL1A2 (NP_000080.2).

Séquence

PTGDPGKNGDKGHAGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPPGPPGFQGLPGPSGPAGEVGKPGERGLHGEFGLPGPAGPRGERGPPGESGAA

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC52128

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

129 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Alpha 2 collagen type I, Alpha 2 type I collagen, Alpha 2 type I procollagen, Alpha 2(I) collagen, Alpha 2(I) procollagen, Alpha-2 type I collagen, CO1A2_HUMAN, Collagen alpha 2(I) chain, Collagen alpha-2(I) chain, Collagen I alpha 2 polypeptide, Collagen of skin tendon and bone alpha 2 chain, Collagen type I alpha 2, OI4, Osteogenesis imperfecta type IV, Type I procollagen

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-COL1A2 Antibody [ARC52128] (A308262)

Western blot analysis of extracts of Mouse bone, using Anti-COL1A2 Antibody [ARC52128] (A308262) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-COL1A2 Antibody [ARC52128] (A308262)

Western blot analysis of extracts of Rat skin, using Anti-COL1A2 Antibody [ARC52128] (A308262) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-COL1A2 Antibody [ARC52128] (A308262)

Immunofluorescence analysis of NIH/3T3 using Anti-COL1A2 Antibody [ARC52128] (A308262) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).