Synthetic peptide within amino acids 1025 and the C-terminus of human CRM1 (NP_003391. 1).
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Notes générales
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Anti-CRM1 Antibody (A295552) was used for IP at 6 µg per reaction. CRM1 was also immunoprecipitated using another rabbit anti-CRM1 antibody. For blotting immunoprecipitated CRM1, Anti-CRM1 Antibody (A295552) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Figure 4: Western Blot - Anti-CRM1 Antibody (A295552)
Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Anti-CRM1 Antibody (A295552) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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