Cyclin B1 (phospho Ser147) Cell Based Kit Elisa (A102543)

Nom du produit
Cyclin B1 (phospho Ser147) Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This Cyclin B1 (phospho Ser147) Cell Based ELISA Kit allows for the detection of Cyclin B1 (phospho Ser147) and the effects that certain stimulation conditions have on Cyclin B1 (phospho Ser147) expression in different cell lines. Qualitative determination of Cyclin B1 (phospho Ser147) concentration is achieved by an indirect ELISA format. In essence, the Cyclin B1 (phospho Ser147) is captured by Anti-Cyclin B1 (phospho Ser147) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Cyclin B1 (phospho Ser147) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Cyclin B1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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