Anti-Cyclin D1 Anticorps [ARC0300] (A306339)

Name: Anti-Cyclin D1 Antibody [ARC0300]
Brand: Antibodies.com
SKU: A306339
Price: 470.0 USD
Availability: InStock

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$470

Anticorps monoclonal [ARC0300] de lapin contre Cyclin D1 pour WB, IHC, ICC/IF et IP.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

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+1 (314) 370-6046

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orders@antibodies.com

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Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-Cyclin D1 Antibody [ARC0300]

Description du produit

Rabbit monoclonal [ARC0300] antibody to Cyclin D1.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).

Séquence

ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC0300

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

34 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (10)

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Figure 1: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 2: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of C6 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 9: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 10: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-Cyclin D1 Antibody [ARC0300] (A306339). This Western blot was performed on the immunoprecipitate using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:1000.

Voir plus de photos

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Anticorps monoclonal [DCS-6] de souris contre Cyclin D1 pour Flow Cytometry, IF, WB et IHC-P.

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Anti-Cyclin D1 Anticorps [DCS-6]

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Voir toutes Anti-Cyclin D1 Anticorps

Anticorps monoclonal [ARC0300] de lapin contre Cyclin D1 pour WB, IHC, ICC/IF et IP.

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