Figure 1: Western Blot - Anti-Cyclin K Antibody (A12868)
Western blot analysis of extracts of various cell lines, using Anti-Cyclin K Antibody (A12868) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Figure 2: Immunohistochemistry - Anti-Cyclin K Antibody (A12868)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cyclin K Antibody (A12868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 3: Immunohistochemistry - Anti-Cyclin K Antibody (A12868)
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Cyclin K Antibody (A12868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Figure 4: Immunohistochemistry - Anti-Cyclin K Antibody (A12868)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Cyclin K Antibody (A12868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
