Mouse monoclonal [C-50] antibody to Cytokeratin 5 + 18.
Spécificité
This antibody reacts with Cytokeratin peptides 5 and 18 (58 and 45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Applications
IHC-P, IP, WB, ICC
Recommander des dilutions
IHC-P: 10 µg/ml; Positive Control: human prostate.
Reactivité
Rat, Hamster, Porcine, Sheep, Canine, Bovine, Human, Mouse
Réactivité croisée
This antibody does not cross react with Xenopus or Chicken.
Immunogène
Cytoskeleton preparation from HeLa cells.
Hôte
Mouse
Clonalité
Monoclonal
Clone
C-50
Isotype
IgG1
Conjuguer
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
MW prédit
45 kDa, 58 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Figure 2: Western Blot - Anti-Cytokeratin 5 + 18 Antibody [C-50] (A86386)
Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard. Lane 2: Immunostaining with Anti-Cytokeratin 5 + 18 Antibody (A86386). Lane 3: Immunostaining with Isotype mouse IgG1 control (PPV-06).