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Anti-Cytokeratin 7 Anticorps (A92201)

Nom du produit
Anti-Cytokeratin 7 Antibody
Description du produit
Rabbit polyclonal antibody to Cytokeratin 7.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 240-355 of human Cytokeratin 7 (KRT7) (NP_005547.3).
Séquence
ISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQE
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
51 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CK 7, CK-7, CK7, Cytokeratin-7, D15Wsu77e, K2C7, K2C7_HUMAN, K7, Keratin 7, Keratin 7, type II, Keratin type II cytoskeletal 7, Keratin, 55K type II cytoskeletal, Keratin, simple epithelial, Keratin, simple epithelial type I, K7, Keratin, type II cytoskeletal 7, Keratin-7, Krt2-7, KRT7, MGC11625, MGC129731, MGC3625, Sarcolectin, SCL, Type II mesothelial keratin K7, Type-II keratin Kb7
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of HepG2 cells, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of Rat lung, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201)
Immunofluorescence analysis of human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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