Anti-DDDDK Tag Anticorps [ARC5111-01] (A305669)

Name: Anti-DDDDK Tag Antibody [ARC5111-01]
Brand: Antibodies.com
SKU: A305669

Cliquez pour ouvrir le point de vue élargi

Ce produit est discontinué

Anti-DDDDK Tag Anticorps [ARC5111-01] (A305669) a été abandonné et n'est plus disponible.

Voir toutes Anti-DDDDK Tag Anticorps.

Produits recommandés pour Anti-DDDDK Tag Anticorps

(3)

A85906

16 Références

Anti-DDDDK Tag Anticorps [F-tag-01]

Anticorps monoclonal [F-tag-01] de souris contre DDDDK Tag pour ICC et WB.

A85282

2 Références

Anti-FLAG Tag Anticorps [FG4R]

Anticorps monoclonal [FG4R] de souris contre FLAG Tag pour Dot, ELISA, IP, IS et WB.

(3)

A85906

16 Références

Anti-DDDDK Tag Anticorps [F-tag-01]

Anticorps monoclonal [F-tag-01] de souris contre DDDDK Tag pour ICC et WB.

A85282

2 Références

Anti-FLAG Tag Anticorps [FG4R]

Anticorps monoclonal [FG4R] de souris contre FLAG Tag pour Dot, ELISA, IP, IS et WB.

Spécifications

Nom du produit

Anti-DDDDK Tag Antibody [ARC5111-01]

Description du produit

Rabbit monoclonal [ARC5111-01] antibody to DDDDK Tag.

Applications

WB, ICC/IF, IP, Flow Cytometry

Recommander des dilutions

WB: 1:2,000-1:10,000, ICC/IF: 1:50-1:200, IP: 1:100-1:500, Flow Cytometry: 1:50-1:200

Reactivité

Species Independent

Immunogène

A synthetic peptide corresponding to DDDDK tag.

Séquence

DYKDDDDK

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC5111-01

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

56 kDa / 50 kDa / 46 kDa / 68 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DDDDK epitope tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (8)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293T, 293T transfected with GSK3B Protein, 293T transfected with BCAT2-N Protein and 293T transfected with BCAT2-C Protein, using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293F transfected with COPB2-N Protein and 293F transfected with COPB2-C Protein, using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Western blot analysis of 293T, 293T transfected with IRF1 Protein using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunofluorescence - Anti-DDDDK Tag Antibody [ARC5111-01] (A305669)

Immunofluorescence analysis of 293T-Flag-C and 293T-Flag-N and 293T cells using Anti-DDDDK Tag Antibody [ARC5111-01] (A305669) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).