Anti-DNA PKcs Anticorps [ARC51265] (A308615)

Name: Anti-DNA PKcs Antibody [ARC51265]
Brand: Antibodies.com
SKU: A308615

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Ce produit est discontinué

Anti-DNA PKcs Anticorps [ARC51265] (A308615) a été abandonné et n'est plus disponible.

Voir toutes Anti-DNA PKcs Anticorps.

Spécifications

Nom du produit

Anti-DNA PKcs Antibody [ARC51265]

Description du produit

Rabbit monoclonal [ARC51265] antibody to DNA PKcs.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 3993-4128 of human DNA-PKcs (NP_008835.5).

Séquence

SDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC51265

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

469 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNAPK, DNAPK catalytic subunit, DNPK 1, DNPK1, Hyper radiosensitivity of murine scid mutation, complementing 1, Hyperradiosensitivity complementing 1, mouse, homolog of 1, HYRC, HYRC 1, HYRC1, IMD26, p350, p460, PKRDC, PRKDC, PRKDC_HUMAN, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC 7, XRCC7

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Western blot analysis of various lysates, using Anti-DNA PKcs Antibody [ARC51265] (A308615) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-DNA PKcs Antibody [ARC51265] (A308615)

Immunofluorescence analysis of HeLa using Anti-DNA PKcs Antibody [ARC51265] (A308615) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).