This antibody recognises the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.
Applications
ELISA, WB
Recommander des dilutions
ELISA: 1:10,000 - 1:50,000, WB: 1:1,000 - 1:5,000
Reactivité
Species Independent
Immunogène
Synthetic peptide sequence DYKDDDDK corresponding to the enterokinase cleavage site, conjugated to Keyhole Limpet Haemocyanin. Residues of cysteine and glycine were used to facilitate coupling at the C-terminus end.
Séquence
DYKDDDDK
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
HRP
Concentration
1 mg/ml after reconstitution.
Forme du produit
Lyophilized
Reconstitution
Reconstitute with 0.1 ml deionised water. For long term storage at -20°C add glycerol to a final concentration of 50%.
Formulation
Supplied in Phosphate Buffered Saline with 0.01% Gentamicin Sulphate and 1% BSA.
Stockage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Notes générales
Rabbit anti DYKDDDDK Tag antibody recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus of target proteins.Rabbit anti DYKDDDDK Tag antibody has been tested against both the immunogen and recombinant proteins containing the DYKDDDDK sequence in ELISA and Western blotting and shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence than the clones M1, M2 and M5, in the majority of assays.In Western blotting of bacterial extracts Rabbit anti DYKDDDDK Tag antibody does not cross-react with any endogenous proteins.
Synonymes
DDDDK epitope tag, DDDDK tag, DDDK, ddk, DYKDDDDK, DYKDDDDK epitope tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG, FLAG tag
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Produits alternatifs à Anti-DYKDDDDK Tag Anticorps (HRP) (A282562)