Anti-eIF4E Anticorps (A13922)

Name: Anti-eIF4E Antibody
Brand: Antibodies.com
SKU: A13922
Price: 470.0 USD
Availability: InStock

Cliquez pour ouvrir le point de vue élargi

$470

Anticorps polyclonal de lapin contre eIF4E pour WB, IHC, ICC/IF et IP.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Mots clés :

Anticorps Validé par Knockout

Spécifications

Nom du produit

Anti-eIF4E Antibody

Description du produit

Rabbit polyclonal antibody to eIF4E.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).

Séquence

MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEP

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

25 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AUTS19, CBP, eIF 4E, EIF 4F, eIF 4F 25 kDa subunit, eIF-4E, eIF-4F 25 kDa subunit, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E_HUMAN, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of various lysates, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Western Blot - Anti-eIF4E Antibody (A13922)

Western blot analysis of extracts from wild type(WT) and eIF4E knockout (KO) 293T(KO) cells, using Anti-eIF4E Antibody (A13922) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 4: Immunohistochemistry - Anti-eIF4E Antibody (A13922)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-eIF4E Antibody (A13922) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.