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Anti-Fascin Anticorps (A13733)

Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com
Anticorps Validé par Knockout
$470
Product Datasheet

Anticorps polyclonal de lapin contre Fascin pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Fascin Antibody
Description du produit
Rabbit polyclonal antibody to Fascin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human Fascin/Fascin/FSCN1 (NP_003079.1).
Séquence
FIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
50 kDa / 55 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
55 kDa actin bundling protein, 55 kDa actin-bundling protein, Actin bundling protein, actin bundling protein, 55-KD, FAN 1, FAN1, Fascin 1, Fascin actin bundling protein 1, Fascin homolog 1, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus), Fascin, sea urchin, homolog of, 1, Fascin1, FLJ38511, FSCN 1, FSCN1, FSCN1_HUMAN, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), Singed drosophila homolog like, Singed like (fascin homolog sea urchin), Singed like (fascin homolog sea urchin) (Drosophila), Singed like protein, Singed, drosophila, homolog of, Singed-like protein, SNL, Strongylocentrotus purpuratus
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts of various cell lines, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts from normal (control) and Fascin/Fascin/FSCN1 knockout (KO) HeLa cells, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Fascin Antibody (A13733)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-Fascin Antibody (A13733)
Immunofluorescence analysis of C6 cells using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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