Anti-Fatty Acid Synthase Anticorps [ARC53122] (A308364)

Name: Anti-Fatty Acid Synthase Antibody [ARC53122]
Brand: Antibodies.com
SKU: A308364

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Ce produit est discontinué

Anti-Fatty Acid Synthase Anticorps [ARC53122] (A308364) a été abandonné et n'est plus disponible.

Voir toutes Anti-Fatty Acid Synthase Anticorps.

Spécifications

Nom du produit

Anti-Fatty Acid Synthase Antibody [ARC53122]

Description du produit

Rabbit monoclonal [ARC53122] antibody to Fatty Acid Synthase.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, ICC/IF: 1:500-1:1,000

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).

Séquence

QQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC53122

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

273 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Enoyl-[acyl-carrier-protein] reductase, FAS, FASN, FAS_HUMAN, MGC14367, MGC15706, OA 519, Oleoyl-[acyl-carrier-protein] hydrolase, SDR27X1, Short chain dehydrogenase/reductase family 27X member 1, [Acyl-carrier-protein] S acetyltransferase, [Acyl-carrier-protein] S malonyltransferase

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of 293F cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts of Rat liver, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Western blot analysis of extracts from wild type (WT) and Fatty Acid Synthase (FASN) knockout (KO) HeLa cells, using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 4: Immunofluorescence - Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364)

Confocal imaging of HeLa cells using Anti-Fatty Acid Synthase Antibody [ARC53122] (A308364), at a dilution of 1:1000, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.