Anti-FGFR1 Anticorps [ARC53424] (A306276)

Name: Anti-FGFR1 Antibody [ARC53424]
Brand: Antibodies.com
SKU: A306276

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Ce produit est discontinué

Anti-FGFR1 Anticorps [ARC53424] (A306276) a été abandonné et n'est plus disponible.

Voir toutes Anti-FGFR1 Anticorps.

Spécifications

Nom du produit

Anti-FGFR1 Antibody [ARC53424]

Description du produit

Rabbit monoclonal [ARC53424] antibody to FGFR1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:2,000-1:10,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic phosphorylated peptide around Y653 & Y654 of human FGFR1 (NP_075598.2).

Séquence

IDYYKK

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC53424

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

122 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Basic fibroblast growth factor receptor 1, bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR 1, FGFR-1, FGFR1/PLAG1 fusion, FGFR1_HUMAN, fibroblast growth factor receptor 1, FLG, FLT-2, FLT2, Fms-like gene, Fms-like tyrosine kinase 2, fms-related tyrosine kinase 2, HBGFR, heparin-binding growth factor receptor, HH2, HRTFDS, hydroxyaryl-protein kinase, KAL2, N-SAM, OGD, Proto-oncogene c-Fgr

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (6)

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Figure 1: Western Blot - Anti-FGFR1 Antibody [ARC53424] (A306276)

Western blot analysis of U-251MG, using Anti-FGFR1 Antibody [ARC53424] (A306276) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-FGFR1 Antibody [ARC53424] (A306276)

Western blot analysis of various lysates, using Anti-FGFR1 Antibody [ARC53424] (A306276) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-FGFR1 Antibody [ARC53424] (A306276)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-FGFR1 Antibody [ARC53424] (A306276) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-FGFR1 Antibody [ARC53424] (A306276)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-FGFR1 Antibody [ARC53424] (A306276) at a dilution of 1:500(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.