Anti-FOXP3 Anticorps (A82808)

Anti-FOXP3 Antibody (A82808) (1µg/ml) staining of Human Muscle (A), (2µg/ml) MOLT4 (B) and (1µg/ml) negative control Pancreas (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-FOXP3 Antibody (A82808) (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells.

Anti-FOXP3 Antibody (A82808) Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-FOXP3 Antibody (A82808) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing strong localization to nucleoplasm. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml).