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Anti-GFAP Anticorps [ARC0206] (A307282)

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Anticorps monoclonal [2A5] de souris contre GFAP pour WB, ICC/IF et IHC.
Nom du produit
Anti-GFAP Antibody [ARC0206]
Description du produit
Rabbit monoclonal [ARC0206] antibody to GFAP.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:500-1:1,000
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GFAP (P14136).
Séquence
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAE
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0206
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
48 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, gfapl, GFAP_HUMAN, Glial fibrillary acidic protein, Intermediate filament protein, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-GFAP Antibody [ARC0206] (A307282)
Western blot analysis of extracts of various cell lines, using Anti-GFAP Antibody [ARC0206] (A307282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-GFAP Antibody [ARC0206] (A307282)
Western blot analysis of extracts of U-251MG cells, using Anti-GFAP Antibody [ARC0206] (A307282) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-GFAP Antibody [ARC0206] (A307282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-GFAP Antibody [ARC0206] (A307282)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-GFAP Antibody [ARC0206] (A307282) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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