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GIT2 Cell Based Kit Elisa (A103294)

Nom du produit
GIT2 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This GIT2 Cell Based ELISA Kit allows for the detection of GIT2 and the effects that certain stimulation conditions have on GIT2 expression in different cell lines. Qualitative determination of GIT2 concentration is achieved by an indirect ELISA format. In essence, the GIT2 is captured by Anti-GIT2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this GIT2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
ARF GAP GIT2, ARF GTPase activating protein GIT2, ARF GTPase-activating protein GIT2, CAT 2, CAT-2, CAT2, Cool associated tyrosine phosphorylated protein 2, Cool interacting tyrosine phosphorylated protein 2, Cool-interacting tyrosine-phosphorylated protein 2, DKFZp686G01261, G protein coupled receptor kinase interacting ArfGAP 2, G protein coupled receptor kinase interactor 2, G protein-coupled receptor kinase-interactor 2, GIT 2, GIT2_HUMAN, GRK interacting protein 2, GRK-interacting protein 2, KIAA0148, MGC760
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - GIT2 Cell Based ELISA Kit (CB6000) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - GIT2 Cell Based ELISA Kit (CB6000)
Western blot analysis of extracts from HepG2 cells, using Anti-GIT2 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - GIT2 Cell Based ELISA Kit (CB6000) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: GIT2 Cell Based ELISA Kit (CB6000)
A diagram showing the protocol for GIT2 Cell Based ELISA Kit.
Protocol Summary - GIT2 Cell Based ELISA Kit (CB6000) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: GIT2 Cell Based ELISA Kit (CB6000)
A summary of the protocol for GIT2 Cell Based ELISA Kit.
Western Blot - Control Antibody - GIT2 Cell Based ELISA Kit (CB6000) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - GIT2 Cell Based ELISA Kit (CB6000)
The mouse monoclonal antibody to GAPDH is used as a positive control inGIT2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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