Anti-Gli1 Anticorps [ARC59108] (A309375)

Name: Anti-Gli1 Antibody [ARC59108]
Brand: Antibodies.com
SKU: A309375

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Ce produit est discontinué

Anti-Gli1 Anticorps [ARC59108] (A309375) a été abandonné et n'est plus disponible.

Voir toutes Anti-Gli1 Anticorps.

Spécifications

Nom du produit

Anti-Gli1 Antibody [ARC59108]

Description du produit

Rabbit monoclonal [ARC59108] antibody to Gli1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant protein of human GLI1.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC59108

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

160 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

GLI, Gli 1, GLI family zinc finger 1, GLI Kruppel family member 1, GLI1_HUMAN, Glioma associated oncogene 1, Glioma associated oncogene homolog, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zfp5, Zinc finger protein GLI 1, Zinc finger protein GLI1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (4)

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Figure 1: Western Blot - Anti-Gli1 Antibody [ARC59108] (A309375)

Western blot analysis of A-549, using Anti-Gli1 Antibody [ARC59108] (A309375) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunofluorescence analysis of Neuro-2a cells using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).