Anti-Glucagon Anticorps [ARC0382] (A309737)

Western blot analysis of extracts of Rat pancreas cells, using Anti-Glucagon Antibody [ARC0382] (A309737) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Anti-Glucagon Antibody [ARC0382] (A309737) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Anti-Glucagon Antibody [ARC0382] (A309737) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat pancreas using Anti-Glucagon Antibody [ARC0382] (A309737) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).