Figure 1: Western Blot - Anti-HA Tag Antibody [AMC0503] (A17312)
Western blot analysis of over-expressed HA-tagged protein in 293T cell using Anti-HA Tag Antibody [AMC0503] (A17312) at 1:10,000 dilution. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Western Blot - Anti-HA Tag Antibody [AMC0503] (A17312)
Western blot analysis of extracts of 293T cells, using Anti-HA Tag Antibody [AMC0503] (A17312) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 3: Western Blot - Anti-HA Tag Antibody [AMC0503] (A17312)
Western blot analysis of extracts of 293T cells, using Anti-HA Tag Antibody [AMC0503] (A17312) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Figure 4: Western Blot - Anti-HA Tag Antibody [AMC0503] (A17312)
Western blot analysis of extracts of 293T cells, using Anti-HA Tag Antibody [AMC0503] (A17312) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 5: Western Blot - Anti-HA Tag Antibody [AMC0503] (A17312)
Western blot analysis of extracts of 293T cells, using Anti-HA Tag Antibody [AMC0503] (A17312) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 6: Immunofluorescence - Anti-HA Tag Antibody [AMC0503] (A17312)
Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells using Anti-HA Tag Antibody [AMC0503] (A17312) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 7: Immunoprecipitation - Anti-HA Tag Antibody [AMC0503] (A17312)
Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using Anti-HA Tag Antibody [AMC0503] (A17312). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
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