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Recombinant Anti-His Tag Anticorps [RM146] (A121323)

Western Blot - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.com Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121223) - Antibodies.com
Anticorps Recombinant
$510

Anticorps monoclonal [RM146] de lapin contre His Tag pour WB, IP, ICC, Flow Cytometry et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

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Anticorps monoclonal [HISH8] de souris contre HHHHHH tag pour Dot, ELISA, IP, IS et WB.
Nom du produit
Anti-His Tag Antibody [RM146]
Description du produit
Recombinant rabbit monoclonal [RM146] antibody to His Tag.
Spécificité
This antibody recognizes recombinant proteins containing the 6 x His Tag or 10 x His Tag, fused to either the amino or carboxy terminus.
Applications
WB, IP, ICC, Flow Cytometry, IHC
Recommander des dilutions
WB: 0.1-1 µg/ml, IP: 0.5-2 µg/ml, ICC: 0.5-2 µg/ml, FC: 0.5-2 µg/ml,IHC: 0.1-1 µg/ml
Reactivité
All Species
Réactivité croisée
There is no cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogène
Mixture containing peptide with His Tag (N terminal) and peptide with His Tag (C terminal).
Hôte
Rabbit
Clonalité
Monoclonal
Clone
RM146
Isotype
IgG
Conjuguer

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-His Tag Antibody [RM146] (A121323)
Western blot of 293T cells, (1) untransfected or (2) transfected with fusion protein X (His tag), and E. coli lysate, (3) without Protein Y (His tag) or (4) with Protein Y (His tag), using Anti-His Tag Antibody [RM146] at 0.2µg/ml.
Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunoprecipitation - Anti-His Tag Antibody [RM146] (A121323)
Immunoprecipitation of 293T cells expressing a His Tag fusion protein by Anti-His Tag Antibody [RM146], and then blotted with Anti-His Tag Antibody [RM146]. (1) Whole lysate control. (2) IP by rabbit IgG control. (3) IP by Anti-His Tag Antibody [RM146].
Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323)
Immunohistochemistry staining of naive HepG2 cells (Negative control) using Anti-His Tag Antibody [RM146].
Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121223) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-His Tag Antibody [RM146] (A121323)
Immunohistochemistry staining of 293T cells expressing nuclear protein X (His tag), using Anti-His Tag Antibody [RM146].

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