Human Angiopoietin-1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human ANG-1 in the samples. A monoclonal antibody specific for human ANG-1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and ANG-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for ANG-1 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of ANG-1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Cell culture supernatant, serum and plasma.
Sensibilité
2.60 pg/ml
Gamme de detection
31.25 - 2000 pg/ml
Spécificité
This kit can assay both natural and recombinant human ANG-1.
Reactivité
Human
Réactivité croisée
This assay has shown no significant cross-reactivity.