Human CCL18 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CCL18 in the samples. A monoclonal antibody specific for human CCL18 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CCL18, and CCL18 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CCL18 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

3.00 pg/ml

Gamme de detection

15.63 - 1000 pg/ml

Spécificité

This kit can assay both natural and recombinant human CCL18.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

Alternative macrophage activation associated CC chemokine 1, Alternative macrophage activation-associated CC chemokine 1, AMAC-1, AMAC1, CC chemokine PARC, CCL18(4-69), CCL18_HUMAN, CKb7, DC-CK1, DCCK1, Dendritic cell chemokine 1, Macrophage Inflammatory Protein 4, MIP-4, MIP4, PARC, Pulmonary and activation regulated chemokine, Pulmonary and activation-regulated chemokine, SCYA18, Small inducible cytokine A18, Small-inducible cytokine A18

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.