Humain Clusterin Kit Elisa (A270347)

Human Clusterin ELISA Kit

Sandwich (quantitative)

This Human Clusterin ELISA Kit applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Clusterin. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Clusterin if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound Clusterin and other components of the sample. A biotinylated Anti-Clusterin detection antibody is then added followed by Streptavidin-HRP. Addition of TMB substrate will result in a colored substrate product. The reaction is stopped with sulfuric acid and the optical density can be quantified using an ELISA plate reader. The concentration of analyte is determined by comparison to a serial dilution of the ELISA standard in parallel.

Microplate

Colorimetric

Serum, plasma, and cell culture supernatant.

0.07-50 ng/ml

Human

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Shipped at 4°C. Store the standard at -20°C; avoid freeze / thaw cycles. Store all other reagents at 2-8°C.

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.