Humain IL-2 Kit Elisa (A323169)

$495
Frais de livraison
Date de livraison
Livraison sous 7-10 jours ouvrables.
Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com
Nom du produit
Human IL-2 ELISA Kit (High Sensitivity)
Description du produit
Human IL-2 ELISA Kit is a high sensitivity sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IL-2 in serum, plasma, and other biological fluids.
Type de test
Sandwich (quantitative)
Principe du test
Human IL-2 ELISA Kit (A323169) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of human IL-2 in serum, plasma, and other biological fluids. An antibody specific for IL-2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-2 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-2 Antibody, which binds the captured IL-2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-2 captured in each well. The concentration of IL-2 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Plate-forme
Pre-coated Microplate (12 x 8 well strips)
Méthode de détection
Colormetric
Instrument
Colorimetric Microplate Reader
Type d'échantillon
Serum, plasma, and other biological fluids.
Sensibilité
0.25 pg/ml
Gamme de detection
0.31-20 pg/ml
Durée du test
3h 30m
Reactivité
Human
Réactivité croisée
No significant cross-reactivity or interference between human IL-2 and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 86% - 100% 91%
EDTA Plasma 8 85% - 98% 90%
Cell Culture Media 8 96% - 109% 101%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 93% - 107% 92% - 107% 90% - 104% 92% - 110%
EDTA Plasma (n=5) 89% - 103% 90% - 103% 87% - 100% 86% - 102%
Cell Culture Media (n=5) 93% - 109% 91% - 108% 92% - 104% 91% - 104%
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonymes
Aldesleukin, IL 2, IL2, IL2_HUMAN, Interleukin 2, Interleukin-2, Interleukin2, Involved in regulation of T cell clonal expansion, Lymphokine , OTTHUMP00000164090, POIL2, T cell growth factor, T-cell growth factor, TCGF
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

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