Principe du test
Human Mkl1 / MRTFA ELISA Kit (A87371) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Mkl1 / MRTFA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Mkl1 / MRTFA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Mkl1 / MRTFA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Mkl1 / MRTFA Antibody, which binds the captured Mkl1 / MRTFA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Mkl1 / MRTFA captured in each well. The concentration of Mkl1 / MRTFA can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.