Human VEGF R2 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human VEGF R2 in the samples. A monoclonal antibody specific for human VEGF R2 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for VEGF R2, and VEGF R2 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of VEGF R2 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plate-forme

Microplate

Méthode de détection

Colorimetric

Type d'échantillon

Cell culture supernatant, serum and plasma.

Sensibilité

0.52 pg/ml

Gamme de detection

62.50 - 4000 pg/ml

Récupération

94% (Average Recovery from Serum Samples).

Spécificité

This kit can assay both natural and recombinant human VEGF R2.

Reactivité

Human

Réactivité croisée

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Stockage

Store at 2 - 8°C.

Synonymes

CD309, CD309 antigen, EC 2.7.10.1, Fetal liver kinase 1, FLK-1, FLK1, FLK1, mouse, homolog of, Kdr, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR 2, VEGFR-2, VEGFR2, VGFR2_HUMAN

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.