Anti-Iba1 Anticorps [ARC51847] (A307825)

Name: Anti-Iba1 Antibody [ARC51847]
Brand: Antibodies.com
SKU: A307825

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Ce produit est discontinué

Anti-Iba1 Anticorps [ARC51847] (A307825) a été abandonné et n'est plus disponible.

Voir toutes Anti-Iba1 Anticorps.

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Anti-Iba1 Anticorps

Anticorps polyclonal de lapin contre Iba1 pour WB, ICC/IF et IHC.

Spécifications

Nom du produit

Anti-Iba1 Antibody [ARC51847]

Description du produit

Rabbit monoclonal [ARC51847] antibody to Iba1.

Applications

WB, IHC, ICC/IF

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivité

Human, Mouse, Rat

Immunogène

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (NP_001614.3).

Séquence

MSQTRDLQGGKAFGLLKAQQEERLDEINKQFLDDPKYSSDEDLPSKLEGFKEKYMEFDLNGNGDIDIMSLKRMLEKLGVPKTHLELKKLIGEVSSGSGETFSYPDFLRMMLGKRSAILKMILMYEEKAREKEKPTGPPAKKAISELP

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC51847

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

17 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AIF 1, AIF-1, Aif1, AIF1 protein, AIF1_HUMAN, Allograft inflammatory factor 1, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, Ionized calcium-binding adapter molecule 1, IRT 1, IRT1, Microglia response factor, MRF1, Protein g1

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (10)

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Figure 1: Western Blot - Anti-Iba1 Antibody [ARC51847] (A307825)

Western blot analysis of extracts of various cell lines, using Anti-Iba1 Antibody [ARC51847] (A307825) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Iba1 Antibody [ARC51847] (A307825)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Iba1 Antibody [ARC51847] (A307825) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Iba1 Antibody [ARC51847] (A307825)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Iba1 Antibody [ARC51847] (A307825) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Iba1 Antibody [ARC51847] (A307825)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-Iba1 Antibody [ARC51847] (A307825) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.